Restriction Enzyme Digest & Gel Electrophoresis of DNA demonstrates how DNA can be specifically cut into fragments by restriction enzymes and then can be separated by fragment size on an agarose gel. Students use lambda DNA and different restriction enzymes to prepare four different DNA digestion patterns.

Key Topics:

  • DNA Structure
  • Restriction Enzyme Function and Use in Cloning
  • Principles of Gel Electrophoresis

This field trip is categorized as an Intermediate field trip, and is appropriate for students at a high school level or above who have had exposure to the concepts of molecular biology. Students that have had experience in the laboratory with micropipettes will be at a distinct advantage when they explore concepts that are fundamental to the way in which modern biotechnology works!

Length of Field Trip: 2.5 to 3 hours

Cost of Field Trip: $144 for up to 16 students; $9 for every additional student; combine with Genetic Transformation & Bioluminescence for a great value! We can also take this field trip On the Road to you.

laboratory protocol
Background information