Gene Expression and Cell Analysis

Molecular Approaches to Neuroscience

June 15-19, 2020

REGISTER

This five-day intensive course offers participants the opportunity to develop skills in performing common molecular biology techniques. Students will benefit from the expertise of highly qualified scientist-instructors and researchers who represent both academia and industry. In addition to laboratory work, students will receive technical and theoretical lectures as well as supplementary guest lectures intended to offer additional perspectives on applications for the techniques learned throughout the week.

Offered with generous support from Promega Corporation
Coordinators

BioPharmaceutical Technology Center Institute
Neuroscience Training Program, UW-Madison
Research & Development and Technical Support Services, Promega Corporation

COST
  • UW-Madison students and special students admitted to the course and wishing to receive 2 credits will enroll through the University registration system and will remit tuition fees directly to the University.
  • For students who do not wish to receive university credits, a special rate of $1200 is offered. (Documentation of current enrollment in an academic institution is required for confirmation of this special rate.)
  • For company employees, faculty and all others, the fee is $1500.
Requirements
  • Eligibility: Graduate students, biotechnology and pharmaceutical industry employees, postdoctoral candidates and principal investigators with an interest in neurosciences are encouraged to register. Course enrollment is limited to 20 participants.
  • Application:  Submit a registration form.
    If you wish to receive graduate credit through UW-Madison    , please be sure to use the Comment section included in this form to detail your prior experiences using molecular biology and your learning goals for the week, as instructed on the form.

    If you do not wish to receive graduate credit through UW-Madison    , please also share that information with us. Your enrollment in the course will be verified upon receipt of payment. Payment options are included in the registration form.
  • NOTE: UW-Madison Graduate Students wishing to receive credit: Please submit your registration form before May 15, 2020. We will review this document and let you know of your acceptance to the course.

 

Instructors AND STAFF
  • Natalie Betz, Ph.D., University of Wisconsin-Madison
  • Karin Borgh, Ph.D., Biopharmaceutical Technology Center Institute
  • Danette Daniels, Ph.D., Research & Development, Promega Corporation
  • Mary Halloran, Ph.D., Professor, University of Wisconsin-Madison
  • Nadine Nassif, M.S., Research & Development, Promega Corporation
  • Amy Prevost, Ph.D., BioPharmaceutical Technology Center Institute
Content
Lectures:
  • RNA Isolation Techniques and Applications
  • Quantification and Analysis of Nucleic Acids
  • PCR and Reverse-Transcriptase PCR
  • Quantitative PCR Methods
  • Bacterial Cloning/Bacterial Transformation
  • Fluorescent Microscopy
  • Tissue Culture Techniques
  • Immunocytochemistry and ELISA
  • Apoptosis Assays
  • Protein Capture and Purification Techniques Using Tags
  • Functional Proteomics and DNA/protein Interactions
Laboratory Work:
  • Isolate RNA from brain tissue
  • Analyze RNA via spectrophotometry and agarose gel analysis
  • Amplify and analyze regions of mouse brain RNA via RT-PCR
  • Amplify and analyze gene expression in mouse brain via qRT-PCR
  • Transform bacteria via T-Vector cloning system
  • Using Inducible Promoters
  • Apoptosis Assay
  • Western Blot