Emerging Techniques in Protein and Genetic Engineering

July 26-30, 2021

OFFERED IN-PERSON AT THE BTC INSTITUTE ON THE PROMEGA CAMPUS IN FITCHBURG:

Oncology 675-002: Summer 2021 Syllabus

Thank you to our generous sponsor, Promega Corporation!

Designed as a model partnership between the educational and business communities, this five-day laboratory course offers participants the opportunity to continue their learning and skill development. Its intensive, integrated format combines lectures, hands-on lab exercises, and interactive problem-solving sessions. Participants will benefit from the knowledge and expertise of highly qualified scientists-instructors and researchers who represent the worlds of both academia and the private sector. Tapping the talents of invited speakers, evening talks are designed to supplement daytime activities, stimulating dialogue regarding current issues in biotechnology.

Coordinators

BioPharmaceutical Technology Center Institute
Department of Oncology, UW-Madison
Research & Development and Technical Support Services, Promega Corporation

COST
  • UW-Madison students and special students admitted to the course and wishing to receive 2 credits will enroll through the University registration system and will remit tuition fees directly to the University.
  • For students who do not wish to receive university credits, a special rate of $1600 is offered. (Documentation of current enrollment in an academic institution is required for confirmation of this special rate.)
  • For company employees, faculty and all others, the fee is $1800.
Requirements
  • Eligibility: Graduate students, biotechnology and pharmaceutical industry employees, secondary and post-secondary teachers, with a B.S. in either biology or chemistry and a minimum of one advanced course in molecular biology. Course enrollment is limited to 20 participants.
  • Application:  Submit a registration form.
    If you wish to receive graduate credit through UW-Madison    , please be sure to use the Comment section included in this form to detail your prior experiences using molecular biology and your learning goals for the week, as instructed on the form.
    If you do not wish to receive graduate credit through UW-Madison    , please also share that information with us. Your enrollment in the course will be verified upon receipt of payment. Payment options are included in the registration form.
  • NOTE: UW-Madison Graduate Students wishing to receive credit: Please submit your registration form before May 15, 2020. We will review this document and let you know of your acceptance to the course.
Instructors
  • Richard Burgess, Ph.D. (Professor, Department of Oncology, UW-Madison)
  • Amy Prevost, Ph.D. (Director, Scientific Courses, BTC Institute)
Content
Lectures:
  • Review of transcription, translation and epigenetics
  • Designing genome editing experiments; knock-in and knock-out genome edits.
  • Cytotoxicity: apoptosis, necrosis and autophagy
  • Molecular biology and drug discovery
  • Mass spectrometry
  • Bioinformatics
  • Reporter genes and vectors
  • Rapid affinity chromatography methods
  • Patent Law and Intellectual Property
  • Next Generation sequencing
  • Studying post-translational modifications
  • Assessing protein degradation
Laboratory Work:
  • Knock-In genome editing using CRISPR/Cas-9
  • Studying targeted protein degradation using PROTACs
  • Selective and pan assays of epigenetic enzymes
  • Assessing cell health
  • Detecting proteins using luminescent assays
  • Working with mass spectrometry data
  • Extensive discussion of data, data analysis, and application of theories and techniques to the current work of course participants.