SCHEDULE: This is an intensive course which features lectures and lab work at the BTC Institute from 9:00 a.m. – 5:00 p.m., expected to conclude by 3:00 p.m. on Friday.

$1200 – Industry, medical, clinical laboratory professionals
$900 – Undergraduate & graduate students, post-doctoral candidates


  • Natalie Betz, Ph.D., UW-Madison Masters Program in BioTechnology and BTC Institute
  • Barbara Bielec, M.S., K-12 Program Director, BTC Institute
  • Nadine Nassif, M.S., Scientist, Research & Development, Promega Corporation
  • Ryan Olson, B.S., Education Specialist, BTC Institute
  • Amy Prevost, Ph.D., Director, Scientific Courses, BTC Institute


CONTENT: This practical course is for medical technologists and other clinical laboratory professionals who wish to have direct, hands-on experience with basic molecular biology techniques utilized in the clinical laboratory.

Upon completion of this course, consisting of 40 hours of combined lectures and laboratory, students will be able to:

  1. Describe current molecular approaches to disease diagnostics and screening.
  2. Isolate nucleic acid from whole blood using a standard industrial kit.
  3. Amplify genes of interest for diagnostic purposes using PCR and RT-PCR.
  4. Discuss the chemistry and application of high-throughput diagnostic tools such as microarray and luminescent SNP detection.
  5. Perform microarray and microarray analysis.
  6. Perform real time PCR analysis of gene targets.
  7. Complete problem solving exercises to demonstrate knowledge of the course material.
  8. Maintain a professional laboratory notebook for all work completed.
Nucleic Acid Isolation
  • Total RNA isolation from blood (mouse blood is used)
  • DNA isolation from blood or tissue
Nucleic Acid Quantitation
  • By spectrophotometry
Nucleic Acid Analysis
  • Restriction Enzyme Digestion of DNA templates
  • PCR of DNA templates to detect point mutations
  • RT-PCR of RNA templates to detect specific gene translocation
  • DNA staining and agarose gel electrophoresis
  • Microarray and microarray analysis
  • Real time PCR analysis
  • RFLP analysis