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Polymerase Chain Reaction (PCR) for Genetic Screening explains the principles and uses of this very powerful technique. Students set up a set of PCR reactions using different DNA templates. Agarose gel electrophoresis is then performed to visualize the PCR-amplified DNA products. Gels are photographed and used to determine the result of a genetic screening scenario.

Key Topics:

  • DNA Replication and DNA Polymerases
  • PCR Reaction Components and their Functions
  • Gel Electrophoresis and DNA Size Determination

This field trip is categorized as an Intermediate field trip, and is appropriate for students at a high school level or above who have had exposure to the concepts of molecular biology. Students that have had experience in the laboratory with micropipettes will be at a distinct advantage when they explore concepts that are fundamental to the way in which modern biotechnology works!

Length of Field Trip: 2.5 to 3 hours

Cost of Field Trip: $144 for up to 16 students; $9 for every additional student; combine with Genetic Transformation & Bioluminescence for a great value! We can also take this field trip On the Road to you.

laboratory Protocol
Background information
Student scenario