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K-12
Programs
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Biotechnology Field Trips
Academic Year 2007/08
Providing opportunities to engage in hands-on activities,
BTCI offers Biotechnology Field Trips to middle and high
school students and their teachers. Designed as a 1.5-4 hour visit
to the BioPharmaceutical Technology Center, field trip experiences
include laboratory experiments based on molecular biology techniques
and, upon request, a tour of Promega's production facilities.
Field trips will be offered on Tuesdays, Wednesdays, and Thursdays,
between 8:30 AM and 6:00 PM. This years program features
five laboratory field trips: Bioluminescence, Restriction Enzyme Digest
and Gel Electrophoresis of DNA, Immunology, Polymerase Chain
Reaction (PCR), and PCR to Detect Genetically Modified Organisms.
New option: Pay your
fees via credit card on-line.
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BIOLUMINESCENCE |
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Bioluminescence uses click beetle luciferase to demonstrate
the transfer of a gene into cells of another species. After a 30-minute
slide show about bioluminescence, participants go into the laboratory
to perform a simulated genetic transformation, which transfers the
luciferase gene, and the ability to make bioluminescent light, into
bacterial cells. Students will learn about:
- Bioluminescence
- DNA and genetic transformation
- Bacteria and sterile technique
Additional Information:
Lab time: 1.5 hours
Cost: If more than 15 students, $4.00/student; 15
students or less, minimum group charge:$60.00.
Bioluminescence Field Trip goes on the road!
Taken on the road, the cost is $6 per student, with a minimum group
charge of $90; plus
mileage at $.445/mile.
Hotel costs will be added for an overnight stay.
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RESTRICTION ENZYME DIGEST & GEL ELECTROPHORESIS
OF DNA |
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Restriction Enzyme Digest and Gel Electrophoresis
of DNA demonstrates how DNA can be specifically cut into fragments
by restriction enzymes and then can be separated by fragment size
on an agarose gel. The participants will use lambda DNA and four
different restriction enzymes to prepare four different DNA digestion
patterns. Topics discussed will include:
- DNA structure
- Restriction enzyme function and use in cloning
- Principles of gel electrophoresis
Additional Information:
Lab time: 2.5-3.0 hours
Cost: If more than 15 students, $6.00/student; 15 students
or less, $90.00.
Restriction Enzyme Digest & Gel Electrophoresis of DNA field trip
goes on the road!
Taken on the road, the cost is $10 per student, with a minimum group
charge of $150; plus
mileage at $.445/mile.
Hotel costs will be added for an overnight stay.
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IMMUNOLOGY |
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Immunology focuses on the scientific use of
antibodies to detect proteins in the laboratory. Participants purify
antibodies from egg yolks and use them to detect E.coli and
Salmonella proteins on a membrane. Students perform laboratory
procedures to foster their understanding of the following:
- Antibody structure and function
- Protein isolation
- Antibody/antigen interactions
Additional Information:
Lab time: 3.0 hours
Cost: If more than 15 students, $6.00/student; 15 students
or less, $90.00.
Immunology Field Trip goes on the road!
Taken on the road, the cost is $10 per student, with a minimum group
charge of $150; plus
mileage at $.445/mile.
Hotel costs will be added for an overnight stay.
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POLYMERASE CHAIN REACTION (PCR) |
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Polymerase Chain Reaction (PCR) explains the
principles and uses of this very powerful technique.
Each student sets up a PCR reaction using different DNA templates.
While the PCR reaction is running, participants pour agarose gels
and then assay their PCR products on the gels. The gels are
photographed and can be used to determine the sizes of the DNA
products. Concepts covered are:
- DNA replication and DNA polymerases
- PCR reaction components and their functions
- Gel electrophoresis and DNA size determination
Additional Information:
Lab time: 3 hours
Cost: If more than 15 students, $6.00/student; 15 students
or less, $90.00.
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PCR to Detect Genetically Modified
Organisms |
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PCR to Detect Genetically
Modified Organisms
utilizes the polymerase chain reaction to identify genetically
modified corn from a variety of food products including corn chips
and cornmeal. Using a magnetic purification system, students will
isolate DNA from a food product of their choice. Samples will be
amplified using PCR and DNA products will be analyzed using agarose
gel electrophoresis. Concepts covered are:
Genetically Modified Organisms
- DNA Isolation
- PCR reaction components and their functions
- Gel electrophoresis and DNA size determination
Additional Information:
Lab time: 3-4 hours
Cost: If more than 15 students, $7.00/student; 15 students
or less, $105.00.
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Stem Cell Presentation |
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A one hour
presentation/discussion regarding stem cells can be added to any
field trip experience for high school and college students;
fee: $20.00.
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FOR MORE INFORMATION |
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Contact: Barbara Bielec, BTCI K-12 Program
Coordinator
BioPharmaceutical Technology Center Institute (BTCI)
5445 East Cheryl Parkway
Madison, Wisconsin, USA 53711
608-277-2618; FAX:608-273-6992
e-mail: barbara.bielec@btci.org
Go to the K-12 Programs Home
Page
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