Biotechnology Field Trips


	K-12 Programs
	Biotechnology Field Trips Academic Year 2007/08 Providing opportunities to engage in hands-on activities, BTCI offers Biotechnology Field Trips to middle and high school students and their teachers. Designed as a 1.5-4 hour visit to the BioPharmaceutical Technology Center, field trip experiences include laboratory experiments based on molecular biology techniques and, upon request, a tour of Promega's production facilities. Field trips will be offered on Tuesdays, Wednesdays, and Thursdays, between 8:30 AM and 6:00 PM. This year’s program features five laboratory field trips: Bioluminescence, Restriction Enzyme Digest and Gel Electrophoresis of DNA, Immunology, Polymerase Chain Reaction (PCR), and PCR to Detect Genetically Modified Organisms. New option: Pay your fees via credit card on-line.
	BIOLUMINESCENCE
	Bioluminescence uses click beetle luciferase to demonstrate the transfer of a gene into cells of another species. After a 30-minute slide show about bioluminescence, participants go into the laboratory to perform a simulated genetic transformation, which transfers the luciferase gene, and the ability to make bioluminescent light, into bacterial cells. Students will learn about: Bioluminescence DNA and genetic transformation Bacteria and sterile technique Additional Information: Background Protocol Set-up Lab time: 1.5 hours Cost: If more than 15 students, $4.00/student; 15 students or less, minimum group charge:$60.00. Bioluminescence Field Trip goes on the road! Taken on the road, the cost is $6 per student, with a minimum group charge of $90; plus mileage at $.445/mile. Hotel costs will be added for an overnight stay.
	RESTRICTION ENZYME DIGEST & GEL ELECTROPHORESIS OF DNA
	Restriction Enzyme Digest and Gel Electrophoresis of DNA demonstrates how DNA can be specifically cut into fragments by restriction enzymes and then can be separated by fragment size on an agarose gel. The participants will use lambda DNA and four different restriction enzymes to prepare four different DNA digestion patterns. Topics discussed will include: DNA structure Restriction enzyme function and use in cloning Principles of gel electrophoresis Additional Information: Background Protocol Set-up Lab time: 2.5-3.0 hours Cost: If more than 15 students, $6.00/student; 15 students or less, $90.00. Restriction Enzyme Digest & Gel Electrophoresis of DNA field trip goes on the road! Taken on the road, the cost is $10 per student, with a minimum group charge of $150; plus mileage at $.445/mile. Hotel costs will be added for an overnight stay.
	IMMUNOLOGY
	Immunology focuses on the scientific use of antibodies to detect proteins in the laboratory. Participants purify antibodies from egg yolks and use them to detect E.coli and Salmonella proteins on a membrane. Students perform laboratory procedures to foster their understanding of the following: Antibody structure and function Protein isolation Antibody/antigen interactions Additional Information: Background Additional Information for Teachers Protocol Set-up Lab time: 3.0 hours Cost: If more than 15 students, $6.00/student; 15 students or less, $90.00. Immunology Field Trip goes on the road! Taken on the road, the cost is $10 per student, with a minimum group charge of $150; plus mileage at $.445/mile. Hotel costs will be added for an overnight stay.
	POLYMERASE CHAIN REACTION (PCR)
	Polymerase Chain Reaction (PCR) explains the principles and uses of this very powerful technique. Each student sets up a PCR reaction using different DNA templates. While the PCR reaction is running, participants pour agarose gels and then assay their PCR products on the gels. The gels are photographed and can be used to determine the sizes of the DNA products. Concepts covered are: DNA replication and DNA polymerases PCR reaction components and their functions Gel electrophoresis and DNA size determination Additional Information: Background Additional Information for Teachers Genetic Screening Scenario Protocol Set-up Lab time: 3 hours Cost: If more than 15 students, $6.00/student; 15 students or less, $90.00.
	PCR to Detect Genetically Modified Organisms
	PCR to Detect Genetically Modified Organisms utilizes the polymerase chain reaction to identify genetically modified corn from a variety of food products including corn chips and cornmeal. Using a magnetic purification system, students will isolate DNA from a food product of their choice. Samples will be amplified using PCR and DNA products will be analyzed using agarose gel electrophoresis. Concepts covered are: Genetically Modified Organisms DNA Isolation PCR reaction components and their functions Gel electrophoresis and DNA size determination Additional Information: Background Information for Teachers Protocol Set-up Lab time: 3-4 hours Cost: If more than 15 students, $7.00/student; 15 students or less, $105.00.
	Stem Cell Presentation
	A one hour presentation/discussion regarding stem cells can be added to any field trip experience for high school and college students; fee: $20.00.
	FOR MORE INFORMATION
	Contact: Barbara Bielec, BTCI K-12 Program Coordinator BioPharmaceutical Technology Center Institute (BTCI) 5445 East Cheryl Parkway Madison, Wisconsin, USA 53711 608-277-2618; FAX:608-273-6992 e-mail: barbara.bielec@btci.org Go to the K-12 Programs Home Page
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K-12 Programs

Biotechnology Field Trips Academic Year 2007/08

Biotechnology Field Trips
Academic Year 2007/08