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Quantitative PCR (qPCR) to Analyze GMO Content uses quantitative PCR (qPCR) to detect how much of a soybean sample is composed of genetically modified (GM) soy in order to determine whether it can be used in food that is labeled Non-GMO. Students will test DNA from different soybean samples using qPCR, then analyze the amplification curves that are the result of qPCR. From that data, participants will determine whether a food sample is Non-GMO. 

Key Topics:

  • Genetically Modified crops and their use in human food and animal feed
  • qPCR as an analytical tool, compared with traditional PCR

This field trip is categorized as an Advanced field trip, and is geared toward students that are advanced high school level or above. This Field Trip utilizes a more sophisticated set of molecular concepts and asks students to relate those concepts to the world around them. In order to guarantee that your students benefit from these experiences, prior experience with PCR, DNA electrophoresis, and the use of micropipettes is highly recommended.

Length of Field Trip: 5 hours, including a 30 minute lunch break

Cost of Field Trip: $208 for up to 16 students; $13 for every additional student

laboratory protocol
Background information