PCR Field Trip

Setup

Note: Set up 4 students per bench.

I. Equipment needed on each lab bench where students will work:

• 4 PCR protocols (these should not be in sleeves, nor written on)
• 4 goggles
• 4 lab coats
• 1 waste beaker
• 1 box of Kimwipes
• 1 ice bucket
• 1 table top microcentrifuge
• 2 p20 Pipetman
• 2 p200 Pipetman
• 2 yellow tip boxes
• 1 blue tip box
• 2 blotting pads (for pipetting practice)
• 2 tubes of colored practice dye (for pipetting practice)
• 1 bottle of nuclease free water
• 1 dropper bottle of mineral oil
• 1 50ml conical tube containing five clear, thin-walled 0.5ml PCR tubes
• 1 test tube rack containing:
  • 4 Sharpie pens (with at least 1 or 2 fine-point)
  • 5 clear 0.5ml Eppendorf tubes
• 1 tube with 12ul FOTO/Vision^TM DNA stain/loading dye

II. Equipment needed on lab benches where students will run gels:

• 1 gel box for every bench/group (4 students)
• 1 power supply for every 2 gel boxes
• 1.0X sodium borate buffer in a bottle, about 1 L per 2 gel boxes
• 1 250ml flask containing premelted 2% agarose
  (2 g agarose in 100mls 1.0X NaBorate makes enough for 2 gels. Plan for 40ml agarose per gel)
• 1 6-well gel comb for each group
• 1 gel tray for each group
• tape to tape gel trays, if necessary
• 1 box Kimwipes

III. Equipment controlled by Instructor:

• Thermalcycler

IV. Reagents needed in each ice bucket (1 bucket per bench)

*100 bp marker: to prepare marker, dispense 6μl marker from stock tube into a labelled, clear .5ml tube. Add 2μl FOTO/Vision^TM DNA stain to the marker. One tube of marker is sufficient for one group.