GMO PCR Field Trip
Set-up
Materials/Equipment Needed
Materials to Be
Supplied:
Disposable
gloves
Pipettes
Pipette Tips
Table top
centrifuge (capable of 13,000 x g.)
Vortexer
Wizard®
Magnetic DNA Purification System for Food (Promega
Cat. # FF3750)
2ml
microcentrifuge tubes
95% or 100%
Ethanol
70% Ethanol Wash
Solution
Isopropanol
(2-Propanol)
Nuclease-Free
Water MagneSphere®
Technology Magnetic Separation Stand (Promega Cat. # Z5331 or Z5332)
65oC
heat block or water bath
1.5 ml Tubes
0.5 ml Tubes
Thin Walled
PCR Tubes
35S Forward
Primer (10 mM)
35S Reverse
Primer (10 mM)
dNTPs
MgCl2
PCR buffer
Thermal cycler
Agarose
Sodium Borate
Buffer
Electrophoresis Power Supply
Electrophoresis Chamber
Gel Casting
Tray
12 Well Gel
Comb
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