SCIENTIFIC COURSES - Introductory

Introduction to Real Time PCR Technologies

An introduction to real time PCR techniques for a wide audience

Dates

CANCELLED for 2010
9:00am - 4:00pm

Schedule:

Training will consist of an overview of PCR technologies and how to conduct real time PCR experiments using a variety of techniques (Plexor, TaqMan and SYBR green). Participants will conduct hands-on experiments and learn about primer design, experimental design and troubleshooting.

Cost and Requirements

    $750 - Industry, medical, clinical laboratory professionals.

    $600 - Undergraduate & graduate students, post-doctoral candidates.

REGISTRATION FORM

PAYMENT FORM

Content

Goals:

This practical course is for scientists, teachers, laboratory technicians, researchers and any others who would like to learn more about real time PCR reactions, how to analyze results, how to optimize each reaction and how to troubleshoot problems with the technologies.

Objectives:

Nucleic Acid Isolation Isolate and analyze nucleic acid for use in downstream
reactions
PCR Reaction Review end-point PCR as a comparison to real-time techniques
SYBR green Reactions Use SYBR dye to track amplification in a real-time reaction
Discuss primer design, optimization and analysis of data and be
able to do these things for your own experiments
Multiplexing Use various reagents for more specific experiments; learn how
multiple fluorophores can be used to allow researchers to
amplify multiple products in one reaction

Coordinators

  • Karin Borgh, Ph.D., Executive Director, BioPharmaceutical Technology Center Institute
  • Amy Prevost, M.S., Scientific Courses Coordinator, BioPharmaceutical Technology Center Institute

Instructors

  • Natalie Betz, Ph.D., UW-Madison Masters Program in BioTechnology and BTCI
  • Benjamin Krenke, M.S., Genetic Identity, Promega Corporation
  • Nadine Nassif, B.S., Scientist, Research & Development, Promega Corporation
  • Ryan Olson, B.S., Education Specialist, BTCI
  • Amy Prevost, M.S., Scientific Courses Coordinator, BTCI

Cancellation

  • If you have registered for a workshop but cannot attend, you may transfer the registration to a colleague within your organization, or you may transfer to another scheduled workshop within 12 months of the original.  There is no charge for transfers.
  • For cancellations made more than two weeks before the start of the workshop, you will be refunded the tuition fee minus a 5% fee.
  • For cancellations made within the two weeks prior to the workshop start date, half the tuition will be refunded.
  • No refunds will be made for non-attendance or withdrawals after the start of each workshop.

Note: BTCI may cancel or postpone any workshop due to unforeseen circumstances. If this occurs, we will provide full tuition reimbursement.  We are not responsible for any related costs, charges, or expenses including imposed penalties by airlines, hotels, or travel agents.

    • SEE ALSO

    For More Information

    Registration Qs:

    Dr. Karin Borgh
    Executive Director
    BTCI
    608-277-2508
    FAX: 608-273-6992
    E-mail: karin.borgh@btci.org

    Course Content Qs:

    Amy Prevost, M.S.
    Scientific Courses Coordinator
    BTCI
    608-277-2466
    FAX: 608-273-6992
    E-mail: amy.prevost@btci.org