Molecular Technologies for the Clinical Laboratory

Introductory Courses

Sponsored by:
UW-Madison, Clinical Laboratory Science Program
BioPharmaceutical Technology Center Institute

with support from:
Promega Corporation

Offered at BTCI in Madison, Wisconsin

TITLE

Molecular Technologies for the Clinical Laboratory

DATES

January 14-18 and May 12-16, 2008

Schedule: The course will begin with breakfast on Monday at 8:30 a.m. This is an intensive course which features lectures and lab work at BTCI from 9:00 a.m. - 5:00 p.m., expected to conclude by 3:00 p.m. on Friday.

COST

$1000 Industry, medical, clinical laboratory professionals
$750 Undergraduate & graduate students, post-doctoral candidates.

CONTENT

Goal:

This practical course is for medical technologists and other clinical laboratory professionals who wish to have direct, hands-on experience with basic molecular biology techniques utilized in the clinical laboratory.

Objectives:

Upon completion of this course consisting of 40 hours of combined lectures and laboratory, students will be able to:

Describe current molecular approaches to disease diagnostics and screening.
Isolate nucleic acid from whole blood using a standard instustrial kit.
Amplify genes of interest for diagnostic purposes using PCR and RT-PCR.
Discuss the chemistry and application of high-throughput diagnostic tools such as microarray and luminescent SNP detection.
Perform microarray and microarray analysis.
Perform RFLP and compare RFLP to luminescent SNP detection.
Complete problem solving exercises to demonstrate knowledge of the course material.
Maintain a professional laboratory notebook for all work completed.

Nucleic Acid Isolation	Total RNA isolation from blood (mouse blood is used) DNA isolation from blood or tissue
Nucleic Acid Quantitation	By spectrophotometry
Nucleic Acid Analysis	Restriction Enzyme Digestion of DNA templates PCR of DNA templates to detect point mutations RT-PCR of RNA templates to detect specific gene translocation Luminescent SNP assays to detect point mutations DNA staining and agarose gel electrophoresis Microarray and microarray analysis RFLP analysis

COORDINATORS

Karin Borgh, Ph.D., Executive Director, Biopharmaceutical Technology Center Institute
Amy Prevost, M.S., Education Specialist, Biopharmaceutical Technology Center Institute

COLLABORATORS

Stacy Walz , MT(ASCP), Senior Lecturer/Education Coordinator, Medical Technology Program, Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison
Sharon Ehrmeyer, Ph.D., Director, Medical Technology Program, Professor, Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison
Angela Ryan, M.S., Marketing Manager, Molecular Diagnostics, Promega Corporation
Hemanth Shenoi, Ph.D., Product Manager, Molecular Diagnostics, Promega Corporation
Steven Wong, Ph.D., Director, Clinical Toxicology, Medical College of Wisconsin

INSTRUCTORS

Natalie Betz, Ph.D., Senior Applications Scientist, Scientific Application Services, Promega Corporation
Barbara Bielec, M.S., Education Specialist, BioPharmaceutical Technology Center Institute
Terri Grunst, M.S., Applications Scientist, Scientific Applications Services, Promega Corporation
Nadine Nassif, B.S., Scientist, Research & Development, Promega Corporation
Ryan Olson, B.S., Scientist, Research & Development, Promega Corporation
Amy Prevost, M.S., Education Specialist, BioPharmaceutical Technology Center Institute
Tracy Worzella, B.S., MT (ASCP), Applications Scientist, Scientific Applications Services, Promega Corporation

CANCELLATION

If you have registered for a workshop but cannot attend, you may transfer the registration to a colleague within your organization, or you may transfer to another scheduled workshop within 12 months of the original. There is no charge for transfers.
For cancellations made more than two weeks before the start of the workshop, you will be refunded the tuition fee minus a 5% fee.
For cancellations made within the two weeks prior to the workshop start date, half the tuition will be refunded.
No refunds will be made for non-attendance or withdrawals after the start of each workshop.

Note: BTCI may cancel or postpone any workshop due to unforeseen circumstances. If this occurs, we will provide full tuition reimbursement. We are not responsible for any related costs, charges, or expenses including imposed penalties by airlines, hotels, or travel agents.

FOR MORE INFORMATION

Register online; or
For course content, contact: Amy Prevost, Education Specialist
BioPharmaceutical Technology Center Institute (BTCI)
5445 East Cheryl Parkway, Madison,
Wisconsin, USA 53711
608-274-4330 x 2466; FAX: 608-273-6992
e-mail: amy.prevost@btci.org
For more information regarding registration, contact: Dr. Karin Borgh, Executive Director
BioPharmaceutical Technology Center Institute (BTCI)
5445 East Cheryl Parkway
Madison, Wisconsin, USA 53711
608-277-2508; FAX:608-273-6992:
e-mail: karin.borgh@btci.org

Go to the Scientific Courses Home Page