SCIENTIFIC COURSES - Introductory

Molecular Technologies for the Clinical Laboratory

Co-Sponsors:

    UW-Madison, Clinical Laboratory Science Program
    BioPharmaceutical Technology Center Institute

With Generous Support From:

    Promega Corporation

Dates

Two Sessions:

    January 11-15, 2010
    May 10-14, 2010

Schedule:

This is an intensive course which features lectures and lab work at BTCI from 9:00 a.m. - 5:00 p.m., expected to conclude by 3:00 p.m. on Friday.

Cost and Requirements

    $1000 - Industry, medical, clinical laboratory professionals

    $750 - Undergraduate & graduate students, post-doctoral candidates.

REGISTRATION FORM

Content

Goals:

This practical course is for medical technologists and other clinical laboratory professionals who wish to have direct, hands-on experience with basic molecular biology techniques utilized in the clinical laboratory.

Objectives:

Upon completion of this course consisting of 40 hours of combined lectures and laboratory, students will be able to:

  1. Describe current molecular approaches to disease diagnostics and screening.
  2. Isolate nucleic acid from whole blood using a standard instustrial kit.
  3. Amplify genes of interest for diagnostic purposes using PCR and RT-PCR.
  4. Discuss the chemistry and application of high-throughput diagnostic tools such as microarray and luminescent SNP detection. 
  5. Perform microarray and microarray analysis.
  6. Perform real time PCR analysis of gene targets.
  7. Complete problem solving exercises to demonstrate knowledge of the course material.
  8. Maintain a professional laboratory notebook for all work completed. 
    Nucleic Acid Isolation
    • Total RNA isolation from blood (mouse blood is used)
    • DNA isolation from blood or tissue
    Nucleic Acid Quantitation
    • By spectrophotometry 
    Nucleic Acid Analysis
    • Restriction Enzyme Digestion of DNA templates
    • PCR of DNA templates to detect point mutations 
    • RT-PCR of RNA templates to detect specific gene translocation
    • DNA staining and agarose gel electrophoresis
    • Microarray and microarray analysis
    • Real time PCR analysis
    • RFLP analysis

Coordinators

  • Karin Borgh, Ph.D., Executive Director, BioPharmaceutical Technology Center Institute
  • Amy Prevost, M.S., Scientific Courses Coordinator, BioPharmaceutical Technology Center Institute

Collaborators

  • Sharon Ehrmeyer, Ph.D., Director, Medical Technology Program, Professor, Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison
  • Stacy Walz , MT(ASCP), Senior Lecturer/Education Coordinator, Medical Technology Program, Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison
  • Steven Wong, Ph.D., Director, Clinical Toxicology, Medical College of Wisconsin

Instructors

  • Natalie Betz, Ph.D., UW-Madison Masters Program in BioTechnology and BTCI
  • Barbara Bielec, M.S., K-12 Course Coordinator, BTCI
  • Martin Ensenberger, Ph.D., Scientist, Research & Development, Promega Corporation
  • Nadine Nassif, B.S., Scientist, Research & Development, Promega Corporation
  • Ryan Olson, B.S., Education Specialist, BTCI
  • Amy Prevost, M.S., Scientific Courses Coordinator, BTCI

Cancellation

  • If you have registered for a workshop but cannot attend, you may transfer the registration to a colleague within your organization, or you may transfer to another scheduled workshop within 12 months of the original.  There is no charge for transfers.
  • For cancellations made more than two weeks before the start of the workshop, you will be refunded the tuition fee minus a 5% fee.
  • For cancellations made within the two weeks prior to the workshop start date, half the tuition will be refunded.
  • No refunds will be made for non-attendance or withdrawals after the start of each workshop.

Note: BTCI may cancel or postpone any workshop due to unforeseen circumstances. If this occurs, we will provide full tuition reimbursement.  We are not responsible for any related costs, charges, or expenses including imposed penalties by airlines, hotels, or travel agents.

    • SEE ALSO

    For More Information

    Registration Qs:

    Dr. Karin Borgh
    Executive Director
    BTCI
    608-277-2508
    FAX: 608-273-6992
    E-mail: karin.borgh@btci.org

    Course Content Qs:

    Amy Prevost, M.S.
    Scientific Courses Coordinator
    BTCI
    608-277-2466
    FAX: 608-273-6992
    E-mail: amy.prevost@btci.org