Theory and Practice of Quantitative Proteomics

Intermediate Courses

Co-sponsored by:
BTCI
UW-Madison Human School of Medicine and Public Health Human Proteomics Program
UW-Madison Department of Chemistry
University of Wisconsin Biotechnology Center
Promega Corporation

Offered at BTCI in Madison, Wisconsin

TITLE

Theory and Practice of Quantitative Proteomics

DATES

October 7-10, 2008

2008 Schedule:

Course Schedule
Schedule	Instructors	Description
Tuesday, October 7, 2008
8:30am – 9:00am Meet students & instructors 9:00am-9:30am Welcome/Introductions	J. Walker, UW-Madison Human Proteomics Program B. Bulleit, Promega Corporation	Welcome and introduction; arrive and obtain course materials Discuss course goals, content and structure. Group leaders will be introduced.
9:30am – 10:15am Lecture	M. Vestling, UW-Madison Department of Chemistry	Introduction to Mass Spectrometry and Proteomics
10:15am – 12:30pm Laboratory Roundtable discussions	N. Betz, & A. Prevost, Biopharmaceutical Technology Center Institute Group Leaders	Separate into four groups to begin lab exercises. SDS-PAGE and protein digestions. Group leaders will lead round table discussions of students’ interests and learning objectives.
12:30pm– 1:30pm Lunch (provided at BTCI)
1:30pm – 2:15pm Lecture: Isotope Labeling	A. Harms, UW-Madison Biotechnology Center	Overview of quantitative proteomics using stable isotope techniques
2:00pm- 5:00pm Laboratory Roundtable discussion	N. Betz, & A. Prevost, Biopharmaceutical Technology Center Institute Group Leaders	Destain gels, prepare protein samples for mass spectroscopy. Topic of Roundtable Discussion TBA
5:00p – 5:30p Recap and Preview	J. Walker, UW-Madison Human Proteomics Program	Recap of progress and discussion of goals for Wednesday
Wednesday, October 8, 2008
8:30am – 9:30am Laboratory	N. Betz, A. Prevost, Biopharmaceutical Technology Center Institute	Sample extraction
9:30am – 10:15am Lecture	G. Barrett-Wilt, UW Biotechnology Center	Overview of modern mass spectrometry instrumentation
10:15am – 12:30pm Laboratory	N. Betz, & A. Prevost, Biopharmaceutical Technology Center Institute	Sample extraction and speed vac
12:30pm – 1:30pm Lunch (provided at BTCI)
1:30pm – 2:15pm Lecture	K. Resing & TBD, University of Colorado-Boulder	Data Anaysis and Interpretation
2:15 pm- 4:00pm Computer Laboratory 4:00pm-4:30pm Recap and Preview	K. Resing, University of Colorado-Boulder M. Vestling, UW-Madision Department of Chemistry A. Harms, UW-Madison Biotechnology Center J. Walker,UW-Madison Human Proteomics Program	database searches Roundtable discussion and goals for Thursday
4:30pm-5:30pm Computer Laboratory	K. Resing, University of Colorado-Boulder M. Vestling, UW-Madision Department of Chemistry A. Harms, UW-Madison Biotechnology Center	Continue with computer exercises
Thursday, October 9, 2008
8:30am-9:30am Laboratory or other activity	TBD, affiliation	TBD
9:30am – 10:15am Lecture	K. Resing & colleague,University of Colorado - Boulder	Spectral counting
10:15am-12:30 pm Computer Laboratory	K. Resing & colleague, University of Colorado-Boulder	Spectral counting software
12:30pm-1:30 pm Lunch (provided at BTCI)
1:30pm – 2:15pm Lecture	N. Kendrick, Kendrick Labs	Carrier ampholine 2D gel phosphoprotein Western blotting
2:15pm—4:30pm Computer Laboratory	K. Resing, University of Colorado-Boulder J. Walker,UW-Madison Human Proteomics Program	Data analysis
4:30pm – 5:00pm Recap and Preview	K. Resing, University of Colorado, Boulder J. Walker,UW-Madison Human Proteomics Program	Roundtable discussion Goals for Friday
Friday, October 10, 2008 on campus at UW-Madison
9:00am – 9:45am Lecture	Y.Ge, UW-Madison Human Proteomics Program	Top down mass spectrometry
9:45am-11:00am Tour	M. Vestling, UW-Madision Department of Chemistry A. Harms, UW-Madison Biotechnology Center Y.Ge, UW-Madison Human Proteomics Program C. Scarlett, UW-Madison School of Pharmacy	See the UW Madison Campus mass spectrometry facilities
11:00am – 12:30pm Laboratory	Group Leaders	Data analysis
12:30pm – 1:30pm Lunch (provided at UW-Madison)
1:30pm – 3:00pm Laboratory	Group Leaders	Data analysis and preparation of group presentations
3:00pm – 4:30pm Lecture/Discussion	Small Groups: Students and Leaders	Group presentations
4:30pm – 5:30pm Closing Session – Reception		Wrap up, evaluation, discussion, suggestions

COST & REQUIREMENTS

$1200 Faculty, industry, medical, clinical laboratory professionals
$1000 Graduate students, post-doctoral candidates.
This includes course materials, lab supplies, breakfast, lunch, drinks and snacks. Participants are responsible for their own lodging, transportation and dinner arrangements; a list of recommended hotels and restaurants will be provided.

Other Requirements:

Eligibility: Proficiency in basic laboratory and computer skills is strongly recommended.
Confirmation: Registration will be confirmed based on receipt of payment or guarantee of payment.

CONTENT

Goals & Objectives:
This hands-on laboratory course will consist of ~80% hands-on work and ~20% theoretical lectures. The students will be divided into four sub-groups in order to cover the four leading quantitative proteomics methods: ITRAC, ¹⁸O labeling, ¹³C acetylation or methylation and spectral counting. Data analysis and interpretation will be emphasized.

Technical Program:
This course will take place both at BTCI and UW-Madison and will be hands-on laboratory intensive.

INSTRUCTORS

Gregory Barrett-Wilt - UW Biotechnology Center

Natalie Betz - BTCI and UW-Madison

Ying Ge - Human Proteomics Program at UW-Madison School of Medicine and Public Health

Amy Harms - UW Biotechnology Center

Nancy Kendrick - Kendrick Labs

Amy Prevost - BTCI

Katherine Resing - University of Colorado-Boulder

Cameron Scarlett - UW-Madison School of Pharmacy

Jeff Walker - Director of the Human Proteomics Program at UW-Madison School of Medicine and
Public Health

Martha Vestling - Department of Chemistry at UW-Madison

CANCELLATION

If you have registered for a workshop but cannot attend, you may transfer the registration to a colleague within your organization, or you may transfer to another scheduled workshop within 12 months of the original. There is no charge for transfers.
For cancellations made more than two weeks before the start of the workshop, you will be refunded the tuition fee minus a 5% fee.
For cancellations made within the two weeks prior to the workshop start date, half the tuition will be refunded.
No refunds will be made for non-attendance or withdrawals after the start of each workshop.

Note: BTCI may cancel or postpone any workshop due to unforeseen circumstances. If this occurs, we will provide full tuition reimbursement. We are not responsible for any related costs, charges, or expenses including imposed penalties by airlines, hotels, or travel agents.

FOR MORE INFORMATION

Register Online
For more information regarding registration, contact: Amy Prevost
BioPharmaceutical Technology Center Institute (BTCI)
5445 East Cheryl Parkway
Madison, Wisconsin, USA 53711
608-277-2466; FAX:608-273-6992
e-mail: amy.prevost@btci.org

Go to the Scientific Courses Home Page

Intermediate Courses

2008 Schedule:

Other Requirements:

Technical Program: This course will take place both at BTCI and UW-Madison and will be hands-on laboratory intensive.

Technical Program:
This course will take place both at BTCI and UW-Madison and will be hands-on laboratory intensive.