SCIENTIFIC COURSES - Intermediate

Molecular Approaches to Neuroscience: Gene Expression and Cell Analysis

Co-Sponsors:

    BioPharmaceutical Technology Center Institute (BTCI)
    Neuroscience Training Program, UW-Madison
    Research & Development and Technical Support Services, Promega Corporation

With Generous Support From:

    Promega Corporation

Dates

June 7-11, 2010
8:30am - 6:00pm

Cost and Requirements

Cost:

  • UW-Madison students and special students admitted to the course will enroll through the University registration system and will remit tuition fees directly to the University.
  • For students who do not wish to receive university credits, a special rate of $900 is offered. (Documentation of current enrollment in an academic institution is required for confirmation of this special rate.)
  • For company employees, faculty and all others, the fee is $1,150.

Requirements:

  • Eligibility: Graduate students, biotechnology and pharmaceutical industry employees, postdoctoral candidates and principal investigators with an interest in neurosciences are encouraged to register. Course enrollment is limited to 20 participants. Please complete a application form.
  • Students wishing to receive credit: Please submit your application materials before April 30, 2010. We will review these materials and let you know of your acceptance to the course on or before May 7, 2010, which will give you enough time to enroll for the summer session.
  • Application: Students wishing to receive graduate credit are required to submit a letter of interest, clarifying eligibility and professional need for course content. This letter can be e-mailed or faxed to Dr. Karin Borgh (karin.borgh@btci.org; fax: 608-273-6992).
  • Confirmation: Registration will be confirmed based on receipt of payment or guarantee of payment.

Content

Goals and Objectives:

This five-day intensive course offers participants the opportunity to develop skills in performing common molecular biology techniques. Students will benefit from the expertise of highly qualified scientist-instructors and researchers who represent both academia and industry. In addition to laboratory work, students will receive technical and theoretical lectures as well as supplementary guest lectures intended to offer additional perspectives on applications for the techniques learned throughout the week.

Lectures:

  • RNA Isolation Techniques and Applications
  • Quantification and Analysis of Nucleic Acids
  • PCR and Reverse-Transcriptase PCR
  • Quantitative PCR Methods
  • Bacterial Cloning/Bacterial Transformation
  • Fluorescent Microscopy
  • Tissue Culture Techniques
  • Immunocytochemistry and ELISA
  • Apoptosis Assays
  • Protein Capture and Purification Techniques Using Tags
  • Functional Proteomics and DNA/protein Interactions

Laboratory Work:

  • Isolate RNA from brain tissue
  • Analyze RNA via spectrophotometry and agarose gel analysis
  • Amplify and analyze regions of mouse brain RNA via RT-PCR
  • Amplify and analyze gene expression in mouse brain via qRT-PCR
  • Transform bacteria via T-Vector cloning system
  • Using Inducible Promoters
  • Apoptosis Assay
  • Western Blot

Coordinators

  • Karin Borgh, Ph.D., Executive Director, BioPharmaceutical Technology Center Institute
  • Amy Prevost, M.S., Scientific Courses Coordinator, BioPharmaceutical Technology Center Institute

Instructors

  • Natalie Betz, Ph.D., University of Wisconsin-Madison MS in Biotechnology Program
  • Martin Ensenberger, Ph.D., Research & Development, Promega Corporation
  • Danette Hartzell, Ph.D., Research & Development, Promega Corporation
  • Natasha Karassina, M.S., Research & Development, Promega Corporation
  • Georgyi Los, Ph.D.,  Research & Development, Promega Corporation
  • Nadine Nassif, M.S., Research & Development, Promega Corporation
  • Martha O'Brien,Ph.D.,  Research & Development, Promega Corporation
  • Amy Prevost, M.S., BioPharmaceutical Technology Center Institute
  • Jolanta Vidugiriene, Ph.D., Research & Development, Promega Corporation
  • Kate Zhao, Ph.D., Research & Development, Promega Corporation
  • Chad Zimprich, B.S., Research & Development, Promega Corporation

Cancellation

  • If you have registered for a workshop but cannot attend, you may transfer the registration to a colleague within your organization, or you may transfer to another scheduled workshop within 12 months of the original.  There is no charge for transfers.
  • For cancellations made more than two weeks before the start of the workshop, you will be refunded the tuition fee minus a 5% fee.
  • For cancellations made within the two weeks prior to the workshop start date, half the tuition will be refunded.
  • No refunds will be made for non-attendance or withdrawals after the start of each workshop.

Note: BTCI may cancel or postpone any workshop due to unforeseen circumstances. If this occurs, we will provide full tuition reimbursement.  We are not responsible for any related costs, charges, or expenses including imposed penalties by airlines, hotels, or travel agents.

    • SEE ALSO

    For More Information

    Registration Qs:

    Dr. Karin Borgh
    Executive Director
    BTCI
    608-277-2508
    FAX: 608-273-6992
    E-mail: karin.borgh@btci.org

    Course Content Qs:

    Amy Prevost, M.S.
    Scientific Courses Coordinator
    BTCI
    608-277-2466
    FAX: 608-273-6992
    E-mail: amy.prevost@btci.org