| Monday, July 26 2010 | Activity/Instructor | Description |
| 8:30a - 9:00a | Welcome and Introductions A. Honigman |
Dr. Honigman will provide a course introduction and welcome students to the BTC Institute |
| 9:00a - 11:30a | Student Introductions All Students |
Students will present (in 10-15 minutes) their work to the group by way of introduction. |
| 11:30a - 12:30p | Lecture A. Honigman |
Transcription |
| 12:30p - 1:30p | Lunch | |
| 1:30p - 3:30p | Lectures A. Honigman, H. Rosen |
Translation |
| 3:30p - 4:30p | Laboratory A. Prevost, N. Betz |
Transfect cells Here's an additional lecture on transfection that we won't cover fully in class |
| 4:30p - 5:15p | Lecture D. Hartzell |
Overview: Studying protein/DNA interactions |
| 5:15p - 6:30p | Lecture H. Rosen |
non-coding RNA |
| Tuesday, July 27 2010 | ||
| 8:30a - 12:30p | Lecture/Discussion M. Urh Laboratory |
Introduction to HaloCHIP technologies; Detecting transcription factors with tagged constructs Review experimental
procedure and practical aspects of using HaloCHIP technology. |
| 12:30p - 1:30p | Lunch | |
| 1:30p - 2:30p | Lecture A. Honigman, H. Rosen |
Vectors and reporter genes |
| 2:30p - 5:00p | Laboratory J. Mendez, N. Murphy, A. Prevost |
HaloCHIP experiment: Wash and Reversal of Crosslinks |
| 5:00p - 6:30p | Lecture D. Casimir |
Patent Law and Intellectual Property |
| Wednesday, July 28 2010 | ||
| 8:30a - 9:30p | Laboratory A. Prevost, D. Czarniecki |
HaloCHIP experiment:DNA purification |
| 9:30a - 10:30a | Lecture K. Reece |
qPCR technologies |
| 10:30a - 11:30a | Lecture D. Hartzell |
Choosing PCR primers, How to use microarrays in your research, and High Throughput Screening |
| 11:30a - 12:30p | Lunch | |
| 12:30p - 1:30p | Lecture J. Mendez |
Protein pull-down experiments |
| 1:30p - 2:30p | Laboratory A. Prevost, K. Reece |
HaloCHIP experiment: Set up qPCR from purified DNA using primers for Jun and Fos |
| 2:30p - 4:00p | Lecture Y. Ge |
Mass Spectrometry Techniques - no online version available |
| 4:00p -6:30p | Guest Presentation B. Mandel |
Biotechnology Resources at UW Madison and New Business Startup Initiatives |
| Thursday, July 29 2010 | ||
| 8:30a - 9:30a | Discussion A. Honigman, H. Rosen |
Review data generated from qPCR |
| 9:30a - 12:30p | Laboratory J. Mendez, N. Murphy |
Protein pull-down |
| 12:30p - 1:30p | Lunch | |
| 1:30p - 3:00p | Lecture S. Goueli |
Kinases as therapeutic targets |
| 3:00p - 4:30p | Laboratory J. Mendez, N. Murphy |
Silver stain gels and perform analysis |
| 4:30p - 6:30p | Reception | Happy hour at the Continental Fitchburg! |
| Friday, July 30 2010 | ||
| 8:30a - 10:30a | Discussion/Lecture A. Honigman, H. Rosen |
Additional topics in Protein and Genetic Engineering |
| 10:30a - 12:00p | Lecture E. Strauss |
Bioinformatics |
| 12:00p - 1:00p | Lunch | |
| 1:00p - 2:30p | Lecture R. Burgess |
Affinity purification of Fluorescently Tagged Proteins |
| 2:30p - 3:00p | Course closing and wrap up Group Photo (Thanks Joe!!) |
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