Emerging Techniques in Protein and Genetic Engineering

Co-Sponsors:

BioPharmaceutical Technology Center Institute (BTCI)
University of Wisconsin - Madison, Department of Oncology

With Generous Support From:

Promega Corporation
Fotodyne, Inc.

Dates

July 26-30, 2010
8:30a - 6:30p

Cost and Requirements

Cost:

• UW-Madison students and special students admitted to the course will enroll through the University registration system and will remit tuition fees directly to the University.
   
• For students who do not wish to receive university credits, a special rate of $1,200 is offered. (Documentation of current enrollment in an academic institution is required for confirmation of this special rate.)
   
• For company employees, faculty and all others, the fee is $1,500.

For students in categories (2) and (3), registration for those admitted to the course will be confirmed based on the receipt of payment, or guarantee of payment.

For all participants, the fee for each designated category includes: lecture/lab documentation; lab supplies; and, breakfast and lunch at BTCI on scheduled course days.

Participants are responsible for their own lodging, transportation and dinner expenses; a list of suggested hotels and restaurants will be provided.

Requirements:

• Eligibility: Graduate students, biotechnology and pharmaceutical industry employees, secondary and post-secondary teachers, with a B.S. in either biology or chemistry and a minimum of one advanced course in molecular biology.
   
• Application: In addition to completing an application form, graduate students wishing to receive UW credit are required to submit a letter of interest, clarifying eligibility and professional need for course content. This letter can be e-mailed or faxed to Dr. Karin Borgh (karin.borgh@btci.org; fax: 608-273-6992). Deadline: May 7, 2010. 
   
• Enrollment: Course enrollment is limited to 18 participants, with approval for enrollment to be granted by Dr. Karin Borgh, based upon review of application materials. Successful applicants will be informed by May 14th.
   

• PAYMENT for Non-UW Participants: Your fees may be paid by credit card on-line, or by a check to "BTC Institute" - 5445 E. Cheryl Pkwy, Madison, WI 53711.

Content

Goals and Objectives:

Designed as a model partnership between the educational and business communities, this six-day laboratory course offers participants the opportunity to continue their learning and skill development. Its intensive, integrated format combines lectures, hands-on lab exercises and interactive problem-solving sessions. Participants will benefit from the knowledge and expertise of highly qualified scientists-instructors and researchers who represent the worlds of both academia and the private sector. Tapping the talents of invited speakers, evening talks and round table discussions are designed to supplement daytime activities, stimulating dialogue regarding current issues in biotechnology.

2009 Content

Lectures:

• Review of basic concepts of molecular biology, protein and genetic engineering
• Transfection and Transformation
• Expression vectors, stable expression, transient expression, controlled expression
• RNA stability, regulation of translation
• Eukaryotic transcription, promoters and post-transcriptional processing
•  siRNA
• Mass spectrometry
• Reporter genes and vectors
• Patent Law and Intellectual Property
• Protein purification techniques

Laboratory Work:

• ChIP
• Quantitative PCR
• Protein pull-down and analysis
• Protein expression in vitro
• Analysis of DNA-protein interactions
• Extensive discussion of data, data analysis and application of theories and techniques to the current work of course participants.

Linked Schedule 2010

Instructors

• Dr. Richard Burgess (Professor, Department of Oncology, UW-Madison)
• Dr. Alik Honigman (Professor, Hebrew University Medical School)
• Dr. Haim Rosen (Professor, Hebrew University Medical School)