Date/Time

Event/Topic

Instructor

MONDAY 07.19.10

8:30a – 9:00a

Welcome and Introductions

All instructors are invited to attend.

Continental Breakfast will be served.

Flow chart presented of work to be done throughout the week

9:00a – 9:30a

Lecture: Overview of lab work- chalk talk about PCR, restriction enzyme digestion and cloning.

N. Betz

9:30a – 11:00a

Laboratory: RT-PCR amplification of RNA target; ligation of instructor generated target

N. Betz, A. Prevost

11:00a – 12:30p

Lecture: PCR variables and PCR technologies

D. Storts

12:30p – 1:30p

Lunch

1:30 – 2:30p
 

2:30p – 3:30p

3:30p – 4:30p

Laboratory: Run gel of RT-PCR product to confirm amplification

Lecture: Bacterial Cloning

Laboratory: Transform cells; prepare plates for overnight

N. Betz, A. Prevost

J. Harnett

N. Betz, A. Prevost

4:30p – 6:30p

Guest Lecture: Enzymes for Molecular Biology

After lecture: plate bacterial cells

M. Slater

TUESDAY 07.20.10

8:30a – 9:00a

Discussion/Laboratory: chalk talk – how to choose colonies; choose colonies for mini-prep (3 ml).

N. Betz, A. Prevost

9:00a – 10:30a

Lecture: RNA and RNA isolation

N. Nassif

*Additional microarray information

10:30a – 12:30p

Laboratory: RNA Isolation; Quantify results using NanoDrop

RNA Isolation Results:
Gels
Spectrophotometry

N. Nassif , A. Prevost

12:30p – 1:30p

Lunch

1:30p – 3:00p

Discussion/Lecture: Real Time PCR Considerations

C. Strayer

3:00 – 4:30p

Laboratory: Set up SYBR reaction and colony PCR

N. Betz, A. Prevost

4:30p – 6:00p

Evening Lecture: Halotag and Purification of CARMI (protein arginine methyl transferase)

Silver Stain Protocol

Methanol Chloroform Precipitation
 

R. Chumanov

WEDNESDAY 07.21.10

8:30a – 11:00a

Lecture: DNA preparation using Pure Yield system

Laboratory: DNA preparation using Pure Yield system

Pure Yield Spectrophotometric Data

D. White, D. Czarniecki

11:00a– 12:30p

Lecture: Reporter Gene Assays

M. Valley

12:30p – 1:30p

Lunch

1:30p – 2:30p

Laboratory:  Analyze results of  qRT-PCR

C. Strayer

2:30p – 4:00p

Lecture/Laboratory: Cell Migration Assays

4:00p – 6:00p

Social Event

THURSDAY 07.22.10

8:30a – 9:30a

AM: Start Wheat Germ Expression

Lecture and Laboratory: Cell migration assays

K. Hulkower, A. Krohn

 
Wheat Germ Expression Results

Chalk Talk: Fusion proteins

Laboratory: Fusion protein purification and analysis

Luminescence Results
Group Photo
Gel Results:
     Fluorescent Gel 1
     Fluorescent Gel 2
     Fluorescent Gel 3
     Coomassie Stained Gel 2
     Coomassie Stained Gel 3

Gel Explainer
 

Friday 07.23.10

8:30a – 3:00p

Lecture: Western blots, ICC and ELISA

Laboratory: Western blot

M. Ensenberger

3:00p - 4:00p

Wrap up, discussion and data synthesis, closing

M. Ensenberger

A. Prevost