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The
W.M. Keck Laboratory for Biological
Imaging and the Laboratory for Optical and Computational
Instrumentation at the University of Wisconsin-Madison, in
cooperation with the BioPharmaceutical Technology Center Institute
and Promega Corporation, are pleased to present The
3rd Symposium on Biological Imaging - Multidimensional Biological
Imaging: Approaches and Innovations.
Date
Friday, September 9th,
2005
Location
BioPharmaceutical
Technology Center
5445 East Cheryl Parkway
Madison, WI
53711
Keynote Speaker
Stefan Hell, Department of
NanoBiophotonics, Max Planck Institute for Biophysical Chemistry
Lecturers
Wolfhard Almers, Kevin
Eliceiri, Sanjiv Sam Gambhir, Jennifer Lippincott-Schwartz,
Badrinath Roysam, Ted Salmon, Bruce Tromberg
Workshops
Fluorescence Lifetime Imaging Microscopy
(Becker and Hickl) and
Automated Methods for Light Microscopy
Imaging and Analysis (Molecular Devices
Corporation)
Overview
The ability of imaging technology in
biology to move beyond the generation of static two-dimensional
images of fixed specimens has become increasing important in
understanding the real-time dynamics of biological systems, whether
they are cells in culture or intact organisms. A shift towards
multidimensional in vivo imaging is key not only for the study of
normal structure and function, but also for shedding light on the
pathophysiology of actual or model diseases, the pharmacology of
drugs, as well as the potential toxicity of drugs and environmental
contaminants.
The addition of information about volume to
image data, typically by the collection of multiple XY image planes,
has allowed for the visualization of living structures as they exist
in three dimensions. It has also created the demand for increasingly
sophisticated imaging software to collect, display, and analyze the
large data sets that are generated. Adding the dimension of time to
two- or three-dimensional images introduces similar computational
challenges, but has been invaluable in visualizing and quantifying
protein interaction and transportation, membrane dynamics, nucleic
acid modification, and changes in ion concentration within
cells.
Although there has been substantial progress in
improving the time scale and spatial extent of imaging data
collected, the traditional lateral and axial resolution limits that
are imposed when images are collected with wavelengths of visible
light have remained largely intact. The Symposium keynote address
will be delivered by Stefan Hell. His lecture will describe imaging
techniques recently developed in his laboratory at the Max Planck
Institute for Biophysical Chemistry in Göttingen, Germany that have
exceeded the diffraction limit of visible light, allowing a
substantial increase in the resolution of fluorescence microscopy to
nanometer scale imaging.
The Symposium will also present
other recent advances in multidimensional biological imaging.
Lectures and poster presentations will highlight how these
techniques can be applied in solving a variety of biological
problems, ranging from imaging subresolution structures to intact
human tissue. Topics covered will include: total internal reflection
(TIRF) microscopy, fluorescence resonance energy transfer (FRET),
whole animal molecular imaging, protein tracking with GFP, nanoscale
fluorescence microscopy, multidimensional image analysis, and
near-infrared diffuse optical imaging. In addition, the Symposium
will feature interactive workshops on fluorescence lifetime imaging
microscopy (FLIM) and automated software-based methods for image
acquisition and analysis
Schedule of
Events
| 7:30-8:00 |
Registration and Continental
Breakfast |
| 8:00-8:10 |
Welcome
Ron Kalil, UW-Madison and Bob Bulleit, Promega
Corporation |
| 8:10-10:10 |
Workshops / Poster Session /
Vendor Exhibits |
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Fluorescence Lifetime
Imaging Microscopy
Becker & Hickl
GmbH (8:10-10:00) |
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Automated Methods for Light Microscopy Imaging and
Analysis Using MetaMorph
Molecular
Devices Corporation (8:10-9:00 and 9:10-10:00) |
| 10:10-10:15 |
Break |
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Lectures: Session I
Moderator: Georgyi Los, Promega
Corporation |
| 10:15-11:05 |
Multidimensional Image
Informatics and Visualization
Kevin Eliceiri, LOCI Group,
UW-Madison |
| 11:10-12:00 |
Quantitative Multi-Dimensional Image
Analysis: The FARSIGHT Approach
Badrinath
Roysam, Center for
Subsurface Sensing and Imaging Systems, Rensselaer Polytechnic |
| 12:00-12:45 |
Lunch / Poster Session / Vendor
Exhibits |
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Lectures: Session II
Moderator: Mary Halloran, UW-Madison |
| 12:45-1:35 |
Imaging Dynamics at the Kinetochore Microtubule Interface
Ted
Salmon, Department of Biology, University of North Carolina
at Chapel Hill |
| 1:40-2:30 |
Imaging Exocytosis and Endocytosis by
Total Internal Reflection (TIRF)
Wolfhard
Almers, The Vollum
Institute, Oregon Health & Science University |
| 2:35-3:25 |
Insights Into Cell Compartmentalization and Protein Trafficking
Using GFP Technology
Jennifer Lippincott-Schwartz, Cell Biology
and Metabolism Branch, NIH |
| 3:30-3:50 |
Break |
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Lectures: Session III
Moderator: Patricia Keely, UW-Madison |
| 3:55-4:45 |
The Role of Optics in Breast Cancer Detection
Bruce Tromberg,
Department of Biomedical Engineering, University of California,
Irvine |
| 4:50-5:40 |
Of Mice and Men: Molecular Imaging in Living
Subjects
Sanjiv Sam Gambhir, Molecular Imaging Program at
Stanford, Stanford University |
| 5:40-6:30 |
Social Hour /
Poster Session / Vendor Exhibits (5:30-7:30) |
| 6:30-7:30 |
Dinner |
| 7:30-9:00 |
Keynote Speaker Address
Introduction by John White,
UW-Madison
Fluorescence Nanoscopy: Breaking the Diffraction
Barrier by the RESOLFT Concept
Stefan Hell, Department of
NanoBiophotonics, Max Planck Institute for Biophysical
Chemistry |
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Registration
Attendance at the Symposium will
be limited to the first 250 registrants.
The deadline for advanced registration is August 26, 2005.
Registration Fees
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Corporate
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$175
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$225
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Faculty
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$100
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$125
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Post Doc/Staff
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$70
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$90
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Graduate Student
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$30
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$40
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Undergraduate
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$20
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$25
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Dinner and Keynote Speaker Address: $30 per person (advanced registration required)
On-line Registration Form
On-line Payment Form
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